The PicoShell workflow allows users to sort single cells based on their growth and/or production of biomolecules. In the workflow, individual cells are placed into nanoliter scale spherical compartments that are freely suspendable, hollow, and contain a porous outer shell. Encapsulated cells can be placed into unique environments such as a bioreactor, outdoor pond, etc that are not possible with previous technologies and can be stained at any point during the workflow. The outer shell can also be changed to retain secretions, DNA, etc. PicoShells are also sortable via flow cytometers, enabling users to sort out cells with throughputs of 1 million+ cells per screen. These features allow users to perform cell screens that were not yet previously possible at incredibly high throughputs and enhance yields of a desired biomolecule.
Cell sorting, colony picking, particles, high-throughput cell assays, microfluidics